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Image Search Results


Journal: Nature protocols

Article Title: Monitoring mammalian mitochondrial translation with MitoRiboSeq

doi: 10.1038/s41596-021-00517-1

Figure Lengend Snippet:

Article Snippet: Incubate at 22 °C for 3 h. Reagent Amount (μL) 50% wt/vol PEG 8000 3.5 10× T4 RNA ligase 2 buffer 0.5 20 μM Universal miRNA cloning linker 0.5 T4 RNA ligase 2, truncated K227Q 0.5 Open in a separate window Add 0.5 μL of 5′ deadenylase (NEB) and 0.5 μL of RecJf exonuclease (NEB), and incubate at 30 °C for 45 min. Add 89 μL nuclease-free water to each sample, bringing the total volume to 100 μL.

Techniques: Clone Assay